APPLICATION OF CELLTOOL
BioRam® provides fast and reliable information about your cells within minutes.
Whenever only small sample amounts are present or diffiulties arise with antibody-based markers, i.e. they are not specific enough, not available at all or when time and cost matters, BioRam® is the tool-of-choice. Just 100 cells are suffi cient for reliable results about cells, their behaviour and development. There is no need for cell expansion, sample preparation or antibody staining.
BioRam® saves time and money with every analysis !
Examples: Monitoring of cell stages using Raman analysis
BioRam® could discriminate apoptotic/necrotic and viable control cells.
Raman spectroscopy enables easy analysis and continuous monitoring of cell apoptosis and necrosis in a fast, label-free and non-invasive way.
The accuracy of Raman-based cell discrimination was 99.7% viable versus dead cells, and 92.3% between cell states. Results were confirmed by flow cytometry and fluorescence microscopy.
In addition, BioRam® monitors
influence of different culture media
impact of hypoxic conditions on cell development, differentiation of stem cells towards arterial fibroblasts activation of neuronal precursor cells using TGFß.
reaction of microglial cells upon lipopolisaccharoids or interleukin.
discrimination of activated from non-activated buccal fibroblasts within 3D-matrix mucoderm®
effect of drugs or toxins providing single-cell kinetics within µ-well equipped microfluidic channels
Monitoring is either done by direct cell measurements and/or via analysis of the supernatant providing information about secreted substances.
For further information, please contact the CellTool Customer Service.