Sensitive in-process monitoring of cell development and differentiation
Handling cell cultures is essential for many areas in research and biotech industry.
Here it is of utmost importance to continuously monitor quality, ‘fitness’ and functionality of cells. Currently, most cell analyzing methods depend on utilization of time and cost intense antibody labeling and/or require large cell amounts (more than 1.000,000 cells). In addition, most of them are so-called ‘endpoint analysing’ tests destroying the cells and hindering continuous observation or further use.
You would like to analyze your cells, but ...
BioRam® provides fast and reliable information about your cells within minutes.
Whenever only small sample amounts are present or difficulties arise with antibody-based markers, i.e. they are not specific enough, not available at all or when time and cost matters, BioRam® is the tool-of-choice. Just 100 cells are sufficient for reliable results about cells, their behaviour and development. There is no need for cell expansion, sample preparation or antibody staining.
BioRam® saves time and money with every analysis !
you can‘t get the right antibody?
you don‘t have enough cells?
current analyses are too expensive?
you don‘t want to wait days or weeks for your results?
Examples: Monitoring of cell stages using Raman analysis
BioRam® could discriminate apoptotic/necrotic and viable control cells.
Although apoptosis and necrosis are well known and intensively studied processes, identification of apoptotic and necrotic cells is challenging. Current gold standard methods like immuno-cytological and biochemical assays are invasive and require specific antibodies as well as large numbers of cells. Continuous monitoring is impeded.
In contrast, Raman spectroscopy enables easy analysis and continuous monitoring of cell apoptosis and necrosis in a fast, label-free and non-invasive way.
The accuracy of Raman-based cell discrimination was 99.7% viable versus dead cells, and 92.3% between cell states. Results were confirmed by flow cytometry and fluorescence microscopy.
In addition, BioRam® monitors:
influence of different culture media
impact of hypoxic conditions on cell development,
differentiation of stem cells towards arterial fibroblasts
activation of neuronal precursor cells using TGFß.
reaction of microglial cells upon lipopolisaccharoids or interleukin.
discrimination of activated from non-activated buccal fibroblasts within 3D-matrix
effect of drugs or toxins providing single-cell kinetics within µ-well equipped
Monitoring is either done by direct cell measurements and/or via analysis of the supernatant providing information about secreted substances.
For further information, please contact the CellTool Customer Service.