Animal Cell Biotechnology - Methods and

Invitation to contribute:

Animal Cell Biotechnology "on the potential of

Raman Laser Spectroscopy (BioRAM system) in single cell analysis and quality control of cell products"

April 2020, Tutzing

CellTool would like to thank the editor for the invitation to contribute the chapter on the potential of Raman Laser Spectroscopy (BioRAM system) in single cell analysis and quality control of cell products. CellTool would like also to congratulate all the authors of “Animal Cell Biotechnology Methods and Protocols Book” for this remarkable collection of protocols and state-of-the-art techniques employed in the optimization, production and analysis of cell cultures and biopharma applications. It was our pleasure at CellTool to contribute to this illustrative book by providing a detailed protocol of the complete workflow of Raman-laser-trapping technique (BioRam® microscopic platform), revealing the great potential of this technique in single cell analysis and quality control of cell products.   

The book: Ralf Pörtner (Editor) of Animal Cell Biotechnology - Methods and Protocols 4th edition - Springer Protocols I Humana Press

#springerprotocols #singlecellanalysis #ramanlasertrapping #molecularfingerprinting 


Free Webinar:

"Raman laser trapping applications of single cell analysis and quality of cell-based products"

• Update on Developments etc. on 22nd of April 2020

April 2020, Tutzing

Dear fellow scientists,


in the past we had common projects applying Raman spectroscopy for label-free single cell analysis. We would like to follow up with you on our former scientific exchange and collaboration and inform you about our recent developments in our technology and data analysis software.

Over the recent years, CellTool has optimized its Raman laser trapping platform BioRam® with an increased sensitivity toward single cell analysis. The improvements applied to BioRam® include sample handlings, measurements automation, and addition of fluorescence module. Furthermore, our newly developed software CT-RamSES is equipped with powerful statistical analysis tools such as PCA, LDA, and HCA, in full automation.  All these developments have expanded the analytical potential of BioRam® and opened new venues of applications such as cell type classifications, quality control of cell-based products, monitoring cell-cell and cell-matrix interaction, and detecting stimulated cells.


In times when we are all kept with limited access to our working place and probably working from home we would like to offer you to participate in a webinar addressing this innovative developments and giving you the opportunity to a direct exchange with Q&As.


The webinar will address the following topics:


  • Recent technology advancements of BioRam® and new results applying unique features of Raman trapping


  • Our recently developed software CT-RamSES in a web-based format and powered by strong automatic data analysis tools such as PCA, LDA, and HCA


  • Expanding our land scape of applications from tissue and single cells measurements to the submicron scale of bacteria and extracellular vesicles



Raman laser trapping applications of single cell analysis and quality control of cell-based products



Wednesday, April 22nd, 2020


10:00 am CET




5:00 pm CET


If you are interested in our webinar please register here to join our free webinar in English.

The BioRam® Laser trapping technology

2020, Tutzing

BioRam® Laser trapping technology allows the optical trapping and sorting of single cells in solution and liquid biopsies. The Raman measurements and data analysis of the cells are automatically processed. Raman results provides a multi-omics profiling of the cells, revealing changes in cellular macromolecules such as nucleic acids, proteins, lipids, and carbohydrates. Consequently, BioRam® can monitor biological changes in cells upon differentiation, cell-cell interactions, cells stimulation and in 3D structures such as organoids.

BioRam® Laser trapping technology

CellTool GmbH. (1/20/20). "Press Release: CellTool Appoints Wulf Fischer-Knuppertz as CEO". Tutzing.

2020, Tutzing

Wulf Fischer-Knuppertz has joined CellTool as CEO. He will closely collaborate with Karin Schütze as CSO and the whole CellTool team. Wulf brings in all his experience from many years of leadership positions in the diagnostics industry (Roche Diagnostics), metabolomics (Biocrates) and biomarkers, to make CellTool's ground-breaking cell analysis technology a gold standard in the market. Along with new investors CellTool is determined to strongly grow the business further promote the novel BioRam® platform in life sciences. (...)

CellTool in "European Life Science-Innovation Hubs of the Munich area"

2019, Munich

European Life Science-Innovation Hubs of the Munich area to indicate Munich’s excellence in Scientific Tools & Instruments R&D and businesses.

Interview on page 40.

Read interview

Read full Report

CellTool in China awarded with the First Health Technology Award

2018, China

CellTool was chosen to participate at the 2018 SIWHT, Sino-EU Innovation Week of Health Technology, that was organized by the Beijing YG Venture Capital M&C iCCAMT, International Co-Innovation Center for Advanced Medical Technology.

Co-organizer: Technopark Zurich, Medical Valley GmbH, Wuhan University Healthcare Alliance, Steinbeis, V-Next, ChinaMedCapital, Healthcare Drinks
Diplomatic Institution:Business Sweden, Swissnex
Sponsor: Xuanwu Government of Nanjing, Nanjing Science and Technology Commission,
Xuzhuang High-tech Industrial Development Zone

The 2018 SIWHT, Sino-EU Innovation Week of Health Technology, took place from October 28th to November 2nd, 2018 in the most innovative area of Shenzhen, Shanghai and Nanjing in China. It was a high-level international event aiming to establish communication platforms for European and Chinese health technology entrepreneurs and investors to integrate into China innovation ecosystems and market, and furthermore, to explore possibilities to set up business in China and benefit from the growth of Chinese economy.

A group of highly motivated and innovative CEOs and CSOs from about 20 European start-up companies travelled together  - well organized by Edward Guan, Dr. Hui Wu, Rumyia Chen and other helpers.
Exciting technologies, innovative spirit and highest motivation could be sensed throughout all the professional lectures that intended to convince industry, research institutions as well as the government to buy or invest into the technologies or support expansion.

During an award ceremony CellTool was selected and awarded with the First Health Technology Award.

Mr. Wu stated that this was due to recommendation by the attendees as the spirit and „seriell“ entrepreneurship of Karin & Raimund was well acknowledged in addition to CellTool’s ground breaking technology for cell analysis.

This event was the beginning of an exciting communication and establishment of an innovative network amongst the participants and the organizers with future collaboration projects in China.

Thank you very much to all the initiators and organizers !!


Invitation XIAN Business Wettbewerb in Hamburg

2018, Hamburg

Congratulation to Arthur Ashkin to his Nobel Prize 2018 for Optical Trapping

2018, Tutzing

Now CellTool systems are based on

Two Nobel prize awarded Technologies

Raman Spectroscopy & Optical Trapping

Learning from the master: Karin Schütze met Art Ashkin during her postdoc (1989-1990; funded by a Humboldt Stipendium) at the University of California in Berkeley, Institute Molecular Biology, supervisor Prof. Manfred Schliwa.

She visited Art at the AT&T Bell Labs and together they researched the mechanism of organelle transportation in amoeba – trapping mitochondria and measure escape forces (Ashkin A et al (1990)_Nature).


Furthermore, they worked on laser zona drilling and sperm insertion (Clement-Sengewald at al.). Since then they stayed in loose contact and Art supported their work (Schütze K and Clement-Sengewald A (1994))

Both companies - PALM and CellTool GmbH – founded and headed by Dr. Karin and Raimund Schütze developed high-performance laser based microscope platforms. They combined laser microdissection and Optical Tweezers into tools for pathologists and oncologist as well as sensitive Raman spectroscopy with Optical Trapping for Next generation cell analysis – 

ground breaking technologies bridging the gap between 
high-performance physical technologies with biomedical applications

Their lifework depends on Arts lifework.

When Karin called Art on Tuesday October 6th, to congratulate he immediately remembered us and repeated what he told already about 10 years ago, how genius Raimund (Schütze) is as he succeeded in constructing a stable Optical Trap that works reliable and does not always require alignment after each switch-off/on. What a compliment from a Nobel laureate!! 


„I only now realized what it means successfully combining the even much more complex Raman technology with Optical Tweezers into an easy to use, reliable and sturdy Raman Trapping Microscope platform.“ stated Karin.

Artikel Physics Focus

Artikel Westfalenblatt

Arthur Askhin



Abstract Award

Congratulation to Romy

2018, Bernried

Dr. Romy Kronstein-Wiedemann, Institute of Transfusion Medicine RCBSD North-East - headed by Professor Torsten Tonn - received an abstract award (see pdf) for Raman results achieved with CellTool’s Raman Trapping microscope (see Abstract)


The poster will be presented at the

51. Jahrestagung der Deutschen Gesellschaft für Transfusionsmedizin und Immunhämatologie e.V. (DGTI) 

19.–21. September 2018 • Lübeck (

ISO Certification

2018, Bernried

CellTool successfully has passed the audit and is now certified under ISO 9001 as well as ISO 13485

CellTool GmbH  enters Raman4Clinics

2018, Bernried

CellTool takes part in the Raman4Clinics project. The project pools European expertise to learn how Raman Spectroscopy can be most efficiently used for the clinical diagnostics of body fluids, bacteria, cells and tissues. We are really happy that we can contribute to this great network and expect that the work of our colleagues and us will help a lot of patients in the future. 

If you want to learn more about Raman4Clinics project, click here: 

Raman4Clinics project 

Network partner I  Quality Control Cell Therapy

2018, Bernried


The aim of the network is the initiation and conception of joint developments of innovative methods and devices for the quality control of cell therapies. These innovative technologies and products shall help to cover the most important aspects and parameters of the quality control of cell-based therapeutics and their starting products. To do so, an adaptive course of action for an improved quality control of human cells shall be developed within the network. This does not only include the setup of Standard Operating Procedures (SOPs) and methods of action, but also the development of devices and diagnostics, which can be used for quality control of different cell therapeutics. The goals of the network shall be in unity with the regulatory parameters of EMA (European Medicines Agency) and FDA (Food and Drug Administration). They shall be expressed in a modular way so that they are adaptable for various therapeutic cell types and products.


Ziel des Netzwerkes ist die Initiierung und Konzeption von gemeinschaftlichen Entwicklungen für innovativer Methoden und Geräte für die Qualitätskontrolle bei Zelltherapien. Die in diesem Netzwerk betrachteten innovativen Technologien und Produkte sollen dazu beitragen, die wichtigsten Aspekte und Parameter der Qualitätskontrolle zellbasierter Therapeutika und ihrer Ausgangsprodukte abzudecken. Hierfür soll im Netzwerk die Entwicklung einer adaptierbaren Vorgehensweise zur verbesserten Qualitätskontrolle humaner Zellen, die in zellbasierten Therapien zum Einsatz kommen angegangen werden. Diese Arbeiten beinhaltet nicht nur „Standard Operating Procedures (SOPs) und Handlungsanweisungen, sondern ganz wesentlich auch die Entwicklung von Geräten und Diagnostika, die man im Rahmen der Qualitätskontrolle von unterschiedlichen Zelltherapeutika anwenden kann. Die Netzwerkziele soll sich am regulatorischen Rahmen von EMA (European Medicines Agency) und FDA (Food and Drug Administration) orientieren und so formuliert sein, dass sie modular einsetzbar ist und schnell an unterschiedliche therapeutische Zelltypen/Produkte adaptiert werden kann.

CellTool GmbH enters Asian market

2017, Beijing & Singapore


CellTool is proud to inform that we achieved the first meeting with potential customers in China. Our distribution partner for China, GZ BioMedTec Solutions UG organized a scientific lecture at State Key Laboratory of Microbial Resources, Institute of Microbiology, CAS in Beijing November 15th, 2017 together with their local partner in China, Forward Bioscience.

Dr. Karin Schütze, the founder and CSO of CellTool presented within a lecture the technical principle of our Raman-Trapping-Microscope  and  showed some validated applications selected from results of more than 100 Service-Lab customers. An intense discussion started and created a high interest. Follow up activities will prove seriousness  of their interest.

During her following visit in Singapore she lectured on BioRam® and its huge potential within various biomedical research fields and applications at the Exploration  Theatrette inside the Biopolis, November 17th, 2017 and in the CBIS, the center of bioimaging sciences in Singapore, November 21st, 2017.
CellTool GmbH is entering the Asian market with these two first promising visits.

ATMP Workshop Paul-Ehrlich-Institut

14th November 2017, Berlin

Dr. Karin Schütze gave a lecture:

"Raman Microscopy: Options and limitations for quality control of cellular products"


"Once again thanks indeed for your (speakers) excellent contributions to our workshop.
The feedback I continue to receive from the audience is extremely positive.“

Resumee Laser-World of Photonics

June 2017

Lectureship of CellTools founder and CSO, Dr. Karin Schuetze in Photonics Forum Halle B2 (B2.560) at 15.20h talking about


Photons on duty for health

Resumee :

The Biophotonics panel was well attended and featured an attractive combination of laser focused and tool implementation talks.

"(...) Danke für den faszinierenden Vortrag gestern. (...)"

Matthias Schulze

Director of Marketing

OEM Components and Instrumentation

Next Generation Cell Analysis

21st April 2017

CellTools invited article from the European commission in Brussels demonstrates application possibilities with our BioRam® system within their brochure: Horizon 2020 projects: portal, Issue 12 and is available under:



CellTool created a technology for fast, highly sensitive and label-free analysis of cells based on Raman spectroscopy. Using the Raman microscope BioRam®, single cells are analyzed non-invasively and without the need of stains or labels. Only about 100 cells are sufficient for a complete analysis, which takes less than an hour. Cells are not affected at all remaining available for further use.

Our Special Report: Next generation cell analysis is online and will be accessible to the end of December 2017 under:



CellTool is Finalist of the Berthold Leibinger Innovation Prize 2016

The jury selected eight finalists for the Berthold Leibinger Innovationspreis from 20 applications and nominations. Ten years back Karin and Raimund Schütze were awarded with the first prize: for their invention: Laser Microbeam and Laser Catapult for Single Cell Capture

See our contribution "Zellanalyse neu definiert" in Biophotonik:

Cell analysis re-defined

Despite of a huge amount of different analysis methods, many questions regarding cell applications are still open and can only be answered with enormous efforts. In this respect a newly developed Raman microscopy system can help, as it combines noninvasive cell analysis under physiologic conditions with a data analysis using less than hundred measured cells.

Invitiation from the European commsission in Brussel


CellTool was invited from the European commission in Brussels to describe the various application possibilities of BioRam® within their brochure:

Horizon 2020 projects

BioRam® Introduction

Lab&More – Fitness Test of motile cells

Florian Zunhammer, Dr. Heidi Kremling, Dr. Tanja Buchacher, Dr. Torsten Tonn, Dr. Roland Werk, Dr. Karin Schütze

Analysis of single cells is never easy – and it gets even more difficult when cells are not fixed at a surface but freely moving. In this case, Raman-Trapping-Microscopes arrest motile samples during spectral analysis. These most powerful devices enable novel insights into characteristics and behavior of eukaryotic cells and microorganisms.

Raman spectroscopy is an analytical method solely based on the interaction of molecules with light. Specifically, it generates a vibrational fingerprint, which is unique for every cell type. Optical trapping allows retaining and manipulation of small particles and motile samples. Combining Raman spectral microscopy with optical trapping is a suitable tool to discriminate between bacterial subtypes and to detect cell infection. In addition it offers the possibility for fast and easy quality control of cell based products.



CellTool GmbH

Lindemannstraße 13

82327 Tutzing

 +49 (0) 8158 906400

CellTool GmbH