Label-free and non-invasive quality assurance of cell based product
Dr. Karin Schütze
Lab&More – Fitness Test of motile cells
Florian Zunhammer, Dr. Heidi Kremling, Dr. Tanja Buchacher, Dr. Torsten Tonn, Dr. Roland Werk, Dr. Karin Schütze
Analysis of single cells is never easy – and it gets even more difficult when cells are not fixed at a surface but freely moving. In this case, Raman-Trapping-Microscopes arrest motile samples during spectral analysis. These most powerful devices enable novel insights into characteristics and behavior of eukaryotic cells and microorganisms.
Raman spectroscopy is an analytical method solely based on the interaction of molecules with light. Specifically, it generates a vibrational fingerprint, which is unique for every cell type. Optical trapping allows retaining and manipulation of small particles and motile samples. Combining Raman spectral microscopy with optical trapping allows efficient analysis of motile eukaryotic cells and microorganisms labelfree and nondestructive.
In this article we provide evidence that Raman spectroscopy combined with optical trapping is a suitable tool to discriminate between bacterial subtypes and to detect cell infection. In addition it offers the possibility for fast and easy quality control of cell based products.