Identifying cells from their biophotonic profile
Raman spectroscopy is based on the Raman effect: when a molecule is exposed to laser light, a small fraction is scattered with a shift in frequency compared to the incident light. This shift is highly specific for each molecule – as unique as a fingerprint.
Raman spectroscopy has long been used successfully as a contact-free technique for material characterization. More recently, scientists have demonstrated its applicability to the field of biology and medicine: several research teams have independently shown that the combined Raman spectra of all biopolymers within a cell form distinct clusters according to cell type and state of differentiation and activity.
Mastering the challenges of biological samples
In close collaboration with scientists at the Fraunhofer Institute for Interfacial Engineering and Biotechnology, CellTool have overcome the particular technical challenges that have so far hampered routine use of Raman spectroscopy in biomedical research and development. Biological samples often emit fluorescence which interferes with their weak Raman signals. Furthermore, they require a tolerable wavelength and appropriate physiological conditions during measurement to preserve cell viability.
BioRam® is the first integrated, robust and easy-to-use system that meets all these challenges.